Phenotypic Characterization of Transgenic Wheat Lines Against Fungal Pathogens Puccinia triticina and Fusarium graminearum.
Identifieur interne : 000478 ( Main/Exploration ); précédent : 000477; suivant : 000479Phenotypic Characterization of Transgenic Wheat Lines Against Fungal Pathogens Puccinia triticina and Fusarium graminearum.
Auteurs : Jagdeep Kaur [États-Unis] ; Dilip Shah [États-Unis] ; John Fellers [États-Unis]Source :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2017.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Maladies des plantes, Triticum.
- microbiologie : Maladies des plantes, Triticum.
- Basidiomycota, Dosage biologique, Fusarium, Phénotype, Résistance à la maladie, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- MESH :
- genetics : Plant Diseases, Triticum.
- microbiology : Plant Diseases, Triticum.
- Basidiomycota, Biological Assay, Disease Resistance, Fusarium, Phenotype, Plants, Genetically Modified.
Abstract
Leaf rust (LR) and Fusarium head blight (FHB) caused by Puccinia triticina and Fusarium graminearum, respectively, are among the most damaging fungal diseases challenging wheat production worldwide. Genetic resistance in combination with fungicide application has been the most widely employed approach to combat these fungal pathogens. Alternative approaches that could augment current practices are needed for the control of these devastating pathogens. To that end, we have recently shown that the extracellular expression of antifungal defensin MtDEF4.2 from Medicago truncatula confers resistance to LR. Additionally, we show that expression of this defensin also provides Type II resistance to FHB under controlled growth chamber conditions. These findings have practical applications for control of these important fungal diseases in wheat. Here, we provide details on conducting LR and FHB bioassays of transgenic wheat lines in the growth chamber.
DOI: 10.1007/978-1-4939-7337-8_17
PubMed: 28913807
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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(MeSH)</term><term>Résistance à la maladie (MeSH)</term><term>Triticum (génétique)</term><term>Triticum (microbiologie)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plant Diseases</term><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Maladies des plantes</term><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Triticum</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Basidiomycota</term><term>Biological Assay</term><term>Disease Resistance</term><term>Fusarium</term><term>Phenotype</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Basidiomycota</term><term>Dosage 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Genetic resistance in combination with fungicide application has been the most widely employed approach to combat these fungal pathogens. Alternative approaches that could augment current practices are needed for the control of these devastating pathogens. To that end, we have recently shown that the extracellular expression of antifungal defensin MtDEF4.2 from Medicago truncatula confers resistance to LR. Additionally, we show that expression of this defensin also provides Type II resistance to FHB under controlled growth chamber conditions. These findings have practical applications for control of these important fungal diseases in wheat. Here, we provide details on conducting LR and FHB bioassays of transgenic wheat lines in the growth chamber.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28913807</PMID><DateCompleted><Year>2018</Year><Month>05</Month><Day>21</Day></DateCompleted><DateRevised><Year>2018</Year><Month>08</Month><Day>14</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1940-6029</ISSN><JournalIssue CitedMedium="Internet"><Volume>1679</Volume><PubDate><Year>2017</Year></PubDate></JournalIssue><Title>Methods in molecular biology (Clifton, N.J.)</Title><ISOAbbreviation>Methods Mol Biol</ISOAbbreviation></Journal><ArticleTitle>Phenotypic Characterization of Transgenic Wheat Lines Against Fungal Pathogens Puccinia triticina and Fusarium graminearum.</ArticleTitle><Pagination><MedlinePgn>269-276</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/978-1-4939-7337-8_17</ELocationID><Abstract><AbstractText>Leaf rust (LR) and Fusarium head blight (FHB) caused by Puccinia triticina and Fusarium graminearum, respectively, are among the most damaging fungal diseases challenging wheat production worldwide. Genetic resistance in combination with fungicide application has been the most widely employed approach to combat these fungal pathogens. Alternative approaches that could augment current practices are needed for the control of these devastating pathogens. To that end, we have recently shown that the extracellular expression of antifungal defensin MtDEF4.2 from Medicago truncatula confers resistance to LR. Additionally, we show that expression of this defensin also provides Type II resistance to FHB under controlled growth chamber conditions. These findings have practical applications for control of these important fungal diseases in wheat. 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